![]() Among the benefits of this design, a chimera allows concurrent testing of disparate target regions of a single pathogen or even different organisms and splitting targets between standards A and B enables control cross-validation, facilitating the distinction of control failure from test failure or success. Furthermore, the equally partitioning of target sites between standards A and B enables cross-validation of positive and negative controls, increasing the confidence in test results. This means that for each target used in the molecular test, standard A would act as positive control, while standard B would act as negative control, or vice-versa. In this design, all the target sequences of a molecular test are retrieved and split between groups A and B, which are then joined in tandem to form single chimeric sequences A and B. ![]() Here, we propose a new design strategy for reference standards that uses matched chimeric synthetic standards in accordance with the principle of A/B testing. The use of in vitro synthesis of RNA and DNA standards allows flexibility in control design and tailoring of controls to the diagnostic test and targets. This leads to delays in diagnosis, missed diagnoses and invalidation of correct test results. For example, if the test returns a negative result from the positive control, it could be because (i) the test failed, (2) the reference control failed or (3) a technical issue with the testing platform. However, this failure of either positive or negative controls is difficult to distinguish from the failure of the diagnostic test itself. In the case of RNA standards, the synthetic controls can also undergo degradation over time, and can be contaminated, confounding the interpretation of test results. Reference standards must be validated and proven fit-for-purpose before used in diagnostic tests. However, separate negative controls, without the target sequences, are also required to ensure the specificity of the diagnostic test. These can be used as positive controls to assess sensitivity of the molecular test undergoing evaluation. The recent advent of DNA synthesis enables the rapid development of reference standards as synthetic constructs representing target genetic material. Reference standards are required to validate the performance of any diagnostic test 1. This chimeric reference standard design approach offers extensive flexibility, allowing representation of diverse genetic features and distantly related sequences, even from different organisms. The chimeric A/B standards were assessed using the US Centres for Disease Control real-time RT-PCR protocol, and showed results congruent with other commercial controls in detecting SARS-CoV-2 in patient samples. This enables control and test failures to be distinguished, increasing confidence in the accuracy of results. This design enables cross-validation of positive and negative controls between the paired standards in the same reaction, with identical conditions. Accordingly, a target region that is present in standard A provides a positive control, whilst being absent in standard B, thereby providing a negative control. The chimeric standards constituted target regions for RT-PCR primer/probe sets that are joined in tandem across two separate synthetic molecules. Using a pair of chimeric A/B RNA standards, this allowed incorporation of positive and negative controls into diagnostic testing for the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). However, negative controls are also required to evaluate test specificity. Current reference standards typically represent target genetic material, and act only as positive controls to assess test sensitivity. These are used as controls, and allow measurement and improvement of the accuracy and quality of diagnostic tests. DNA synthesis in vitro has enabled the rapid production of reference standards.
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![]() ![]() The list of Supported OS, Hardware and software. But you can install it in other editions of windows. We will download the SQL Server developer edition / SQL Server Express edition and show you how to install it. In this how-to guide, we learn how to download and install SQL Server 2017 step by step.Once downloaded, run the installation file and follow the prompts. Find the download link (it will read Download SQL Server Management Studio 17.6 or similar, depending on the version at the time of download) and click it to download SSMS. SQL stands for Structured Query Language and is the mechanism by which client applications are. OpenBase SQL is a database server application that provides high-speed access to structured data. If you are looking for MySQL Workbench support please contact Oracle. Please note that SQL Workbench/J has no relation to the product MySQL Workbench which is maintained and owned by Oracle. One great feature of FrostWire is the direct connect option which allows you to directly connect to a friend Os x 10.10 download for mac. When LimeWire was shutdown in 2010 FrostWire took the limelight and its popularity grew with improved performance, stability and new added features. FrostWire is basically an identical application to LimeWire and began as a file sharing client on the Gnutella network later dropping support for Gnutella in version 5 to become a BitTorrent client instead. Development of FrostWire began in September 2004 after LimeWire considered making significant changes to its program due to mounting pressure from the RIAA. Need a software version that is not currently listed?Make a software request now! FrostWire DescriptionįrostWire is a free cross-platform BitTorrent client with an elegant UI providing unlimited connections and downloads with added support for UI customization, online chat, simplified file search, firewall-to-firewall transfers and iTunes integration. ![]() It basically assumes you prefer PDF and allows you several things to do with that PDF. Printing is as easy as clinking a button.After you’ve completed all your tabs, you simply convert/format this to PDF with just one button.Each piece of the script is a separate tab, i.e.Actually, you can even use the ‘tab’ button on the keyboard to flip from between text styles. It has an easy text style drop down menu to help you format it just right.Thanks goodness for user-friendly designs. Here’s the good part: because I have no clue what I’m doing, here are my favorite things about this program that make things less complicated for me: I honestly thought I would have to rewrite the whole thing. I reconverted it fairly easy, it allowed me to simply flip the script style. I mistakenly converted my script to a ‘theater’ format. What I found was that Celtx allows you to write in 7 different script formats including Film, Audio-Visual, Theater, Audio-Play, Storyboard, Comic Book and Novel. Then I dove deeper into the actual functionality of the program. I couldn’t wait for him to write a song about how I was his best mentee ever! He didn’t write a song at all, in fact he said, “That ain’t screenplay format.” (Yes, Ron used “ain’t,” and yes, you have my permission to judge him.) Wait, what? Not the right format? Foiled again. I was so excited, I sent it off to the cast first, uploaded it to Google docs, shared it, etc. Ron would be so proud of me, I might actually get a cyber, buckle-to-buckle man hug! Within 15 minutes I converted my kindergarten script to a grown-up script even my Grandpa would be proud of. Guess what, it was really free! I immediately downloaded the software and converted my script to the ‘real thing’. Open-source software that bills itself the #1 Choice for Media Pre-Production. įeeling like a new, 5-minute old, shiny balloon sat on by a two year-old in a birthday party, I finally landed on Celtx. Who remembers to do that? I’m still trying to cancel my Columbia House subscription from 1989. 30 DAYS! Probably that old trick, call and cancel before the month is up. However, it is no longer free, in fact there are only 2 options to purchase with free use for 30 days. One of my favorite things in this world, ‘free’. (There’s that BIG word again.) He pointed me toward, because it was a good program for the price… free. One that’s easy to read and looks professional. He actually responded very nicely encouraging me to start putting my writing in an actual, commonly used script format. He said, “Phil you stink, why are you using MS Word for scripts dummy!” Or at least, that’s what I heard. As any good mentor would, within a short amount of time, he reviewed and as always responded quickly and honestly. (I know, don’t judge.) I was really proud of a recent piece of mine and sent it off to the godfather to be blessed and get his stamp of approval. I am cheating in a specific area of filmmaking. Not on Ron, I wouldn’t do that, the relationship is still new. I actually think he’s put out more than what I’ve given him I’m considering signing over my first born to him. In return for some key grip work and behind the scenes help, Ron is gracious enough to check, double check and provide me direction on many things involving my new adventure in filmmaking. Basically, without a mentor guiding, challenging, shaping and keeping you accountable, how ‘good’ can you actually get all by yourself? My mentor is in fact, Ron Dawson. Typically I’ve found we limit ourselves when we set out to get better at something and primarily rely on our own methods of accountability and follow-up. In fact, I sought out a filmmaking mentor to help keep me accountable on all aspects of the art. Now, I am on a mission to educate myself on all the moving pieces of filmmaking, photography, the business, casting and writing, etc. (YIPEE! Who knew?) Now, saying that doesn’t mean that I’m good at it, it just means some poor soul paid for my work. I would say I’m an amateur, although I’ve read recently that if you’re making money doing it, you’re actually a professional. It’s no secret that I’m new to filmmaking and videography, but it’s become one of my driving passions in the past 2 years. Phil is the guy with the big, bald and sexy head. Hi, hello, nanoo nanoo, or however these greetings typically should start in a blog. In typical Phil format, this is what he came up with. I asked him to write a review of the pre-production program Celtx. You may remember Phil as “Big Brother” in my 48 Hour Film Project “ The Last Author.” Phil is part of my mentorship program. Phil Stevens - videographer, husband, father, actor and funny man. ![]() Today is a guest blog post from someone who is new to the business. ![]() ![]() Is Gaijin launcher safe?īecause it’s malware: it’s malicious software and it makes your computer work for Gaijin. In 2019, War Thunder was among the most played games on Steam with over 25,000 concurrent players. Golden Eagles are our in-game currency which you can use to purchase: Premium vehicles – increases the earning rate of Research Points and Silver Lions for each battle and comes pre-equipped with all available modifications for that vehicle. Can you buy Golden Eagles with Silver Lions? As Josh Cruz pointed out, the game is not dead but it’s stagnating, and the number of players is also slowly decreasing. Military > Army > Main battle tanks: Countries Compared Introduced in November 2020’s New Power update, many have called the West-German Leopard 2A6 the best tank in War Thunder. Deliveries of the K2 Black Panther tank commenced to the South Korean Army in 2016. Furthermore it is the most expensive main battle tank to date. What is the deadliest tank in the world?Ĭurrently the Black Panther is one of the most advanced main battle tank in the world, outclassing anything North Korea or China have. … Over 700 authentic tanks to choose from among 12 nations. World of Tanks is a global online multiplayer free-to-play game dedicated to tank warfare in the mid-20th century. Will there be a new World of Tanks?ĪpFor the very first time in the series’ history, World of Tanks will be bringing modern tanks to the battlefield – introducing a new way to play for over 21 million tankers! … Our passionate players will finally be able to get their hands on some of the most popular tanks of the modern era. Delete that entire folder.Ĭonclusion: So, with all things considered – is World of Tanks still worth playing in 2021? The answer is a big yes! The game is still one of the most active MMOs that you can get into this year and also one of the most welcoming ones. It should send you to /appdata/local/gaijin folder. Right click on it, and select ‘open file location’. How do you get rid of Gjagent?įor future reference, anyone trying to get rid of gjagent.exe, go to your task manager > startup tab > gjagent. How can I disable Gaijin.Net Updater? Deselect “Autoupdate” in Launcher Settings in War Thunder and Crossout and the app will be instantly disabled. No, the app is designed to only check for updates for War Thunder, Crossout and other Gaijin games (in development). ![]() – Press Windows key and type « %appdata% » and press enter. … It’s worth keeping an eye out for sales in the War Thunder store or on Steam to get the most for your money. The two currencies are Silver Lions, mostly earned from playing missions and battles, and Gold Eagles, mostly bought with real money. Even in contemporary times, it also gave the world some impressive tanks, namely the Leopard 2K. Germany is already one of the most desirable factions to pick in War Thunder thanks to its all-around great selection of tanks. What is the best country for tanks in War Thunder? How do I stop war thunder from opening on startup?.Can you buy Golden Eagles with Silver Lions?.What is the deadliest tank in the world?.How do I get rid of Gaijin net updater?.What is the best country for tanks in War Thunder?.Gold can be obtained via high in-game performance (contests, missions), events, special offers, and direct purchase based on real world money. WoT is P2W because it provides in-game tools for it. Steam uninstall does nothing and it is not visible in control panel either (on the Steam « version »). Apparently the launcher installs itself in multiple places, including hidden folders. Indeed Is Gaijin a malware? because it’s malware: it’s malicious software and it makes your computer work for Gaijin. Unless you want to play helicopters, no, outside of just a handful of vehicles war thunder is not pay to win. You are basically doing « paid marketing tasks », with a cut going to the Author and Gaijin, while the rest go to you in the form of GE. The same applications format exist for other games and the author has a deal with Gaijin. There are no working cheats that may affect the gameplay, only client modifications is possible. ![]() She spent her childhood along Skaneateles Lake, where her father owned the Hunter Dinerant in Auburn and managed the Auburn Community College cafeteria. Like them, Neilia had grown up and attended school in central New York. ![]() She fostered a comfortable space in her classroom, and she easily connected with her students. Neilia was the teacher every student wanted to have, Spooner said. “When she started speaking, she was exactly what my girlfriend had told me: very soft spoken and beautiful personality, and looking,” Spooner said. She was wearing brown-rimmed glasses and had her hair pulled back. On the first day of the school year, a hot summer morning, Spooner took her seat in the middle of the classroom and glanced up at Ms. “Oh, boy, you’re going to have Neilia Hunter?” her friend said. She had received her teacher assignment in the mail. In the summer before seventh grade, 12-year-old Susan Spooner couldn’t wait to go back to her school, Bellevue Heights. But time and again, despite the haziness that comes with the years passing, everyone says, more than anything, she was kind. They recall that she was reserved, naturally beautiful and modest. Those who knew her in Syracuse now remember only bits and pieces about her. ![]() ![]() Neilia - who inspired others to see the best in themselves as a teacher, role model, mother and friend - died in a car accident 50 years ago, on Dec. And a lot of his early success, he has said, is tied to meeting Neilia. He doesn’t need to sneak into four-star resorts anymore. and is arguably SU’s most notable alumnus. They later had three children: Beau, Hunter and Naomi.Īlmost 60 years later, Biden has the highest position in the U.S. Mary’s of the Lake Church in Skaneateles, New York. On the fourth and last day of his trip, he’d admit it: “You know we’re going to get married.” Walking back from their first date later that night, Biden couldn’t shake the thought that Neilia was the one. Nobody ever felt smaller around Neilia,” he wrote. “That was her special touch, the way she made everyone feel okay about themselves. And most of all, he learned that she was sincere and always said the right thing, even during his embarrassing moments. He learned that she hoped to teach junior high school in Syracuse in September. That day, he learned Neilia was from Skaneateles, New York, and she was in her last year at Syracuse University. She was so easy to talk to,” Biden wrote in his 2007 memoir Promises to Keep. “Basically I fell ass over tin cup in love-at first sight. “I’m Neilia Hunter.” She looked up at him with her green eyes and smiled, her face illuminated by the afternoon sun. He walked over and sat at the edge of the woman’s lounge chair. Ultimately, it didn’t matter what side the coin landed on. Fred Sears, the impartial friend of the group, proposed they flip a coin. He wanted to talk to the woman by the pool. The out-of-place college student had snuck in with two friends that spring day in 1964, the men wrapping themselves in the hotel’s beach towels and pretending to be guests. He wasn’t supposed to be there, at the British Colonial Hotel in Nassau, Bahamas. S he made his way to the deck of the swimming pool, Joe Biden noticed the blonde woman sitting in a chaise lounge chair. Get the latest Syracuse news delivered right to your inbox. Essentially, this means that the tomatoes are cooked, strained, and any seeds are removed. Similarly, ketchup is made from tomato concentrate.
Simulated frost depth is defined as the 0 OC isotherm andihsbased on the . The Global Forecast System model is used in this visualization.įound inside – When discrepancy between simulated and measured frost depths does not depend on erroneous snow predictions, it is mainly a result of the soil compartmentalization. Additionally, snow depth is a prognostic variable of numerical weather prediction models.41 Due to the high . This page supplies graphical forecasts from numerical weather models.įound inside – Historical temperature data from weather stations are used to determine the frequency and severity of losses for each crop. For example, computer-forecast models idealize the real . Found inside – Why, for example, will the heavy snow that was predicted sometimes not materialize? For one, computer models have inherent flaws that limit the accuracy of weather forecasts. A: When viewing a loop animation, you may drag the slider handle on a touchscreen to advance through the loop frames. Global models with imagery for the entire world include the ECMWF, GFS, ICON, CMC, NAVGEM, and their associated ensemble prediction systems.įound inside – For example, using an analytical model of climate, snow accumulation and melt, Woods (2009) presented predictions of snowmelt timing and magnitude, as a component of ungauged predictions of seasonal snowpack evolution. ![]() Even solar radiation passing through clouds, and therefore time of day, can have an impact on melting. So, in that regard, it can be more useful for estimating the ground accumulation at the end of a snowstorm than our 10:1 and Kuchera snowfall products. Hovering the time series with the cursor will show the minimum, maximum and mean vaules of the ensemble and the result of the main run.Now, to the topic of the SLR (often informally called “snow ratio” or just “ratio”). Because there are more potential forecast outcomes as you head farther out into the future, ensembles become especially useful after Day 4 or 5. Different ensemble systems have different numbers of ensemble members and the more ensemble members there are, the better the forecast will be as it will take into account a wider range of possibilities. If the ensembles disagree, it’s wise not to put too much confidence in one outcome or another. If all, or almost all, the ensemble members agree on a particular outcome, you can have high confidence that that outcome will occur. ![]() Ensembles are a great tool for gauging uncertainty in a forecast. Each one of these ideas will create its own outcome, known as an ensemble member. Ensembles attempt to fix this problem by starting the model with a bunch of ideas of what the atmosphere could be doing right now. Any small error in the weather model initially due to this gap in observation is compounded exponentially out through time due to chaos. Because we can’t observe every tiny bit of air in our atmosphere, our picture of the weather currently is incomplete. Adfree Plus (with extra features) ExtraĮnsembles are produced by running the same weather models many different times with slightly varying initial conditions.Lake Murray, Ardmore OK (WeatherOK, USA).Tropical cyclone tracks (ECMWF/Ensemble).Forecast Ensemble Heatmaps (Up to 5 models, multiple runs, graph up to 16 days) EXTRA.Forecast Ensemble (Up to 5 models, multiple runs, graph up to 16 days).Forecast XL (Graph and table up to 10 days - choose your model). ![]() 14 day forecast (ECMWF-IFS/EPS, graphs with ranges).Meteograms (Graph 3-5 days - choose your model).Weather overview (Next hours and days, 14 day forecast). ![]() Many of the latter inmates become ‘workers’, who can reduce their sentence by performing jail maintenance or working in the kitchen. Unlike the Florida Department of Corrections, where inmates are sent once convicted of a crime and sentenced to a period of more than a year, the Martin County Jail System is populated by short-term offenders those who have just been arrested and awaiting to be bonded out by friends or family, those who cannot afford or get bailed and are awaiting trial, or those who have already been convicted of a crime and sentenced to a term of less than one year. The goal of such tight security is to keep both the staff and the inmates safe.įor other jails in Martin County, jails in neighboring counties, and local police jails and juvenile detention centers for young offenders under age 18 in this county, click here. Guards at entrances and in areas not frequented by inmates are armed with various firearms. They also carry stun weapons, short clubs, flashlights that have dual use as both a light source and as a weapon, and handcuffs. Guards that circulate in the same general area of the inmates are armed with eye-blinding mace that will turn an inmate having a violent outburst into a weeping child. The Martin County Jail is equipped with a state-of-the-art locking system, closed circuit cameras covering every area where inmates are located, and jail staff that utilize a communication system that keeps each of the guards and civilian staff in constant contact. Therefore, given the mix of potentially dangerous inmates, the security level is very high. Inmates in this jail range from low level misdemeanor offenders to those being held and awaiting trial for violent crimes like robbery, rape, and murder. How to search for an inmate? Can you look up an inmate’s mugshot? How do you look up an offender’s criminal charges, bond or inmate number? How do you visit an inmate? What are the visitation hours? Can you visit inmates in Martin County by video from home? How do you get phone calls from an inmate? Can you send mail, books, or newspapers to an inmate? How do you send an inmate money? Does Martin County Jail have inmate commissary? Can an inmate receive and/or send email or text messages? Do inmates in Martin County have access to computers or tablets? How do you pay an inmate’s bail or bond?Įvery year the Martin County Jail has approximately 13920 bookings, with a daily average of 696 inmates and maintains control of the facility with a staff of 174. You can also get answers to whatever questions about an inmate, and the services for Martin County Jail that you may have by clicking on any of the questions below: ![]() The main reason that people call 77 is to find out if a particular person is in custody, although you can look up an inmate online by going here. While the police in the cities and towns in Martin County can arrest and detain offenders, the Martin County Jail is the facility that is accredited by Florida to hold inmates for more than 72 hours.Ĭan be reached 24 hours a day, 365 days a year at 77. The Martin County Jail is the main jail in Martin County Florida. ![]() ![]() Cheats March 2018 This Kitchen Tool Will Help You Become a Better Chef This Travel-Size Steamer Will Save You Time and Money Fire at UN Warehouse Destroys 50 Tons of Food for Starving Yemenis Report: Trump Freezes $200 Million Pledged for Syrian Recovery Efforts Malala Makes First Visit to Pakistani Hometown Since Taliban Attack Trump Accuses Amazon of Postal ‘Scam,’ Demands Washington Post Register as a ‘Lobbyist’ Cher Appeals to Saudi Crown Prince Mohammad Bin Salman Over Detained Royal Ted Nugent: Parkland’s Student-Activists Are ‘Soulless’ Liars IOC Chief Thomas Bach Meets With Kim Jong Un in Pyongyang Fox Host Laura Ingraham Announces ‘Vacation’ Amid Growing Backlash Russia Orders Dozens More UK Diplomats Out, Expels Diplomatic Staff of 23 Countries These LED Pliers Are What’s Missing From Your Toolbox Page Six: Actress Sanaa Lathan Allegedly Bit Beyoncé’s Chin Police Officer Who Shot Alton Sterling Has Been Fired, New Body Cam Footage Released Stacey Dash Withdraws Her Congressional Bid Pippa Middleton’s Father-in-Law Arrested for Alleged Rape of Minor Two British ISIS Fighters Say Hostage Beheadings Were a ‘Mistake’ Stephon Clark Was Shot Eight Times From Behind and Side, Independent Autopsy Shows Office In Charge of Vetting Trump Appointees Is Inexperienced, Understaffed: Report Report: Congress Looking Into Manafort Associate’s Jet Flight Sheriff ‘Disappointed’ in Outcome of Mateen Wife’s Trial Report: 12 Dead, 500+ Injured in Gaza-Israel Border Protest Kenneth Starr: Stormy Daniels Claims Should be ‘Assessed’ by DOJ Pruitt Paid Lobbyist $50 a Night for Capitol Hill Apartment Mother in Fatal Cliff Crash Admitted Abusing Child in 2010 Stoneman Douglas Student Allegedly Posts Gun and Bullet Pics Police: Cop-Impersonator Suspect Dead After Killing Real Kentucky Officer U.S. Sci-Fi, sports etc) and underneath it you get a small window with the front of the currently selected DVD. The application consists by a vertical strip on the left showing you the different collections you may have (you can add as many collections you want, e.g. The application is written for Mac OS X and it sports a metal interface. But pls.Here is a nice utility helping you catalog your VHS or DVD video disks, DVDpedia. But If I would found something, I prefer to using the search in my own lib and not for a new movie.Īlso I missed sometimes a bit greater OS interface. Personally I would prefer it will create a new entry for a new movie. If you hit the shortcut "cmd + n" it will create a new folder. The only thing who can be a bit better is the handling here and there. This will safe me also sometimes a bit of money or time, because in the past who I using Delicious, I bought sometimes a movie two times in the store. If you fill in the original title of the movie, you also can safe the time to fill in the IMDB ID, but for German movies this will often not work and I'm a German user.Īlso I love to have some own free forms, where I can insert keywords who I prefer and the application will remember this keywords for future filling.Īnd the best thing is, that there is a mobile application available to, to sync my full collection with all the list's who I created. For example: If you fill in the IMDB ID of the movie, you can simply import all the information's of the selected movie in seconds. So the support is gorgeous at ever point where you need help or has suggestions.Īlso I can insert now a lot of more details around my movie collection as I ever can do before and I love the connection to a wide range of different sources to complete the movie informations. The beginning was a bit tricky, because I has problems to import my Library from Delicious Library 2, but after I contact the support over Mail, they helping me in less hours. Now I'm working with DVDPedia for around one year and it works so great for me. run in trouble with using the Amazon API and he dropped the mobile client. Well, there was one in the past, but the dev. The final brake with Delicious I made, as it was more and more clear that they won't develop again an application for iOS. Also the application was slowing down with my growing collection (over 400 DVDs). I go sometimes in contact with the developer over mail or on their "complicated" feedback forum, but nothing has changed over the years. But with the time I wished me some other features like linking to Trailers on my HD or the web like over YouTube. Well, anyway, in my opinion the DVDpedia is good work.Ī long time I was working with Delicious Library 1 & 2 and I was happy with all the given features. All you get is the library that you can also alter a bit on your iPhone, but why would you wanna do that? Makes much more sense to import all the predefined categories too. I thought after the synching I will be able to survey my carefully arranged smart collections. ![]() The only downer is the iPhone app that conveniently is available too.įirst of all you have to pay in addition, that policy is not really up to data any more. Was able to create all kind of smart lists without any trouble in a jiffy. Immediately it showed error free all the contents of my movie collection. Easily imported the database that I exported with HunterExporter. I spontaneously decided to try out DVDpedia today since I experienced a couple of issues with DVDHunter in the last months (very slow, wrong content shown for selected movie, delays in processing tasks, uncomfortable handling of database, faulty smart collections etc.).ĭownloaded DVDpedia and everything works flawlessly. |
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